Orc2 antibody drosophila

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August undefined, 2024

WebDec 19, 2002 · As the Drosophila Myb complex bound to both ACE3 and Ori-β in vitro, we tested whether the Myb complex directly interacted with Orc. Immunoprecipitation from … WebDrosophila provides a powerful model for understanding replication control in metazoans. ... The anti-ORC2 antibodies were provided by Richard Austin and Steve Bell and used at a dilution of 1:2500. Anti-DUP antibodies were used at a dilution of 1:1000. Microscopy for fluorescently labeled embryos and ovaries was done using either a Biorad ...

ORC2 - Wikipedia

WebFeb 15, 2024 · Gene Summary Origin recognition complex subunit 2 ( Orc2) encodes a subunit of the origin recognition complex (ORC), which is essential for the initiation of … WebWe isolated mutations in Drosophila E2F and DP that affect chorion gene amplification and ORC2 localization in the follicle cells. In the follicle cells of the ovary, the ORC2 protein is … novant health covid numbers

A WD-Repeat Protein Stabilizes ORC Binding to Chromatin

WebJun 30, 2009 · We have observed similar staining patterns for other Drosophila ORC subunits, including Orc1 and Orc2. Moreover, ORC was found in the nucleolus in human cells ( 31, 32 ). These findings may indicate a potential interaction of ORC with components of the ribosome biogenesis pathway ( 31, 33 ). WebOct 2, 2001 · Immunostaining of the Drosophila embryos was performed as described by using affinity-purified antibody raised against Drosophila ORC2 and ORC6 proteins. Results Six different mutant complexes and wild-type recombinant ORC were prepared as … how to slow down videos on tiktok

Role for a Drosophila Myb-containing protein complex in …

Identification and Characterization of the Human ORC6 Homolog* - Jou…

WebJul 30, 2008 · We showed previously by coimmunoprecipitation that Drosophila Orc1 and Orc2 proteins interact with the dDP/dE2F1/Rbf1 complex in ovarian extracts, and we wished to further characterize this interaction . ... Anti-HA (Sigma), Anti-ORC2, and anti-dE2F1 antibodies were used at 1∶100 dilutions. Anti- RBF2 was used at 1∶1 dilutions. Anti-V5 ... WebApr 13, 2024 · Cell Research - Dietary cysteine drives body fat loss via FMRFamide signaling in Drosophila and mouse. ... Antibodies were used at the following dilutions: mouse anti-nc82 (1:100, DSHB), rabbit ... novant health covid vaccine schedulingWebDrosophila melanogaster ORC PBS phosphate-buffered saline Initiation of eukaryotic DNA replication takes place at multiple sites in the genome. In yeast Saccharomyces cerevisiae, a six protein origin recognition complex (ORC) 1 binds to ARS consensus sequences in a sequence-specific manner ( 1 , 2 ). novant health covid vaccine hanes mall

"WebAbout 20% of the Orc2 subunit is eluted from the nuclei after an RNase treatment ... anti-rabbit IgG (Amersham) and Alexa Fluor 488-conjugated goat anti-mouse IgG (Molecular Probes) were used as secondary antibodies. Drosophila cell culture extracts. Drosophila Schneider line 2 (S2) cells were maintained at 25°C in Schneider's Insect Medium ... " - Orc2 antibody drosophila

Orc2 antibody drosophila

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WebDec 1, 2024 · To analyze the ability of mutant proteins to interact with the rest of the complex, we immunoprecipitated ORC from obtained transgenic flies using Orc2 … WebNov 2, 2011 · We thank I. Chesnokov and the Bloomington Drosophila Stock Center (Bloomington, IN) for flies, S. Bell and M. Botchan for α-Orc2 antibodies, J. Powers of the Indiana Light Microscopy Imaging Center (Bloomington, IN) for helpful advice, and M. Lilly and members of the Calvi lab for advice and comments on the manuscript. We thank M. …

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WebPreparation of the ORC2 antibodies. The Drosophila ORC2 coding sequence was PCR amplified from a 4- to 8-hr embryonic cDNA library (Brown and Kafatos 1988). The PCR product was subcloned into pCYB2 (New England Biolabs) usingNdeI and SmaI sites engineered into the termini of the PCR product. DmORC2 protein was expressed in … WebRabbit anti-Drosophila melanogaster (Fruit fly) Orc2 Polyclonal antibody: Uniprot No. Q24168: Target Names: Orc2: Alternative Names: Orc2 antibody; CG3041 antibody; Origin …

WebMay 1, 2004 · Antibody to the origin recognition complex subunit 2 (ORC2)recognizes large foci that localize to the endogenous chorion gene loci and to active transgenic constructs … WebContact Cusabio to Customize Orc2 Antibody, which reacts with Drosophila melanogaster (Fruit fly) and will validated for ELISA, WB (ensure identification of antigen). Call: 301-363-4651; ... Rabbit anti-Drosophila melanogaster (Fruit fly) Orc2 Polyclonal antibody: Uniprot No. Q24168: Target Names: Orc2: Alternative Names:

WebOct 8, 2010 · IP with Orc2 antibody followed by immunoblot with ORCA polyclonal antibody (pAb) showed the association of Orc2 with endogenous ORCA (molecular weight of ORCA ∼70kDa; Figure 1 D). Finally, immunoprecipitation with ORCA pAb in human cells clearly demonstrated the association of ORCA with ORC subunits ( Figure 1 E). WebJun 24, 2004 · The centrosomes of HeLa cells arrested in the aberrant mitotic state failed to label with anti-Orc2 antibodies, suggesting that the Orc2 centrosome labeling was specific (Supplementary Figure S10B). ... Mutations in Drosophila Orc2 also disrupt HP1 association with heterochromatin (Huang et al, 1998).

WebGene: orc-2 Immunogen: Recombinant His6-tagged ORC-2 fusion protein Alternate Gene Names: Alternate Antibody Name: 4D4 Clonality: Monoclonal Alternate Antigen Name: …

WebThe newly identified 30-kDa protein hsORC6 is 28% identical and 49% similar to ORC6p from Drosophila melanogaster, which is consistent with the identities and similarities found … novant health covid vaccine locationsWebMouse Anti-D. melanogaster Orc2 Antibody is a mouse antibody against Orc2. It can be used for Orc2 detection in Western Blot, Enzyme-Linked Immunosorbent Assay. … how to slow down voicemail playbackWebDec 14, 2000 · This pattern of staining was also observed using affinity-purified ORC2 antibody and in three Drosophila cell lines following either paraformaldehyde or methanol fixation (Fig. 2 b). ORC2 staining is remarkably similar to that of Xenopus ORC1, which is also absent from metaphase chromosomes but present on anaphase chromatids [33]. It … how to slow down voice recording for typingWebThe origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast … how to slow down water evaporationWebPhosphospecific antibodies were generated using synthetic peptides corresponding to amino acids 112–120 (CELAKpTPQKS) and 221–230 (CPVGKEpTPSKR) of ORC2 for anti … novant health cpeWebThe origin-recognition complex (ORC) has an essential role in defining DNA replication origins [1] and in chromosome segregation [2, 3]. Recent studies in Drosophila orc2 mutants [4–6], and in human cells depleted of ORC2 [3], have suggested that this factor is also implicated in mitotic chromosome assembly. We asked whether ORC was required for M … novant health cpht salaryWebDrosophila Myb recognizes a highly conserved DNA sequence10,11, but the speciﬁc binding properties of the glycerol gradient fractions might be more complex than that of … how to slow down videos on youtube