Thp-1 cells คือ

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August undefined, 2024

WebTHP-1 cells are a human monocyte suspension cell line from peripheral blood of a 1 year old infant who had acute mnocytic leukemia. Web12 Transfer cells + media to a T-25 flask 13 Incubate cells at 37 °C and 5% CO2 and 80% humidity 14 THP-1 cells replicate after ~26 hours. In practice, it takes 2 days for a true …

THP-1 - TIB-202 ATCC

WebCell culture and differentiation The THP-1 cell line was obtained from culture collection of the Chinese Academy of Sciences, Shanghai, China and kept at 1×10^6/ml in RPMI 1640 medium supplemented with 10% heat-inactivated fetal bovine serum(FBS) and 1% penicillin-streptomycin (PS). THP-1 cells were Study of the inflammatory cells response to ... la tilma

THP-1 Cell-based Assays Monocyte Reporter Cell Lines - InvivoGen

WebTHP1-defASC cells are derived from THP-1 human monocytic cells. THP-1 cells are the most commonly used model cell line for the study of inflammasome activation as they … WebTHP-1 is a human leukemia monocytic cell line, which has been extensively used to study monocyte/macrophage functions, mechanisms, signaling pathways, and nutrient and drug … WebA potential trajectory is to use this cell line to study the novel molecular mechanisms in monocytes and macrophages in relation to the physiology and pathophysiology of the … la timeline

Mastering the Art of Growing THP-1 cells - Bitesize Bio

Monocytic THP-1 cells diverge significantly from their primary ...

WebNov 1, 2014 · The THP-1 cell line is isolated from the peripheral blood of a 1-year old male patient suffering from acute monocytic leukemia [7]. This cell line has been widely used to study immune responses while cells are not only in the monocyte state but also in the macrophage-like state [8], [9]. THP-1 macrophages, when stimulated with LPS, expressed ... WebTHP-1. TIB-202 ™. THP-1 is a monocyte isolated from peripheral blood from an acute monocytic leukemia patient. This cell line can be used in immune system disorder … christina stielli sjukdomWeb因此，thp-1是各大实验室常用的急性单核细胞白血病细胞系，是研究免疫和炎症的理想工具。 thp-1 的应用——巨噬细胞分化与炎症模型. thp-1与人原代单核细胞都可以被诱导分化 … christina sullivan duke

"WebThe day of transfection, count the cells to determine culture density. Plate 1 x 10 5 cells per well in 0.5 ml of complete growth medium. Cell density should be 50~80% confluent on the day of transfection. For each well of cells to be transfected, dilute 0.5 μg of DNA into 100 μl of Opti-MEM® I Reduced Serum Medium without serum. " - Thp-1 cells คือ

Thp-1 cells คือ

Standardized protocols for differentiation of THP-1 cells to ...

Although THP-1 cells are of the same lineage, mutations can cause differences as the progeny proliferates. In general, THP-1 cells exhibit a large, round, single-cell morphology. The cells were derived from the peripheral blood of a 1-year-old human male with acute monocytic leukemia. Some of their … See more THP-1 is a human monocytic cell line derived from an acute monocytic leukemia patient. It is used to test leukemia cell lines in immunocytochemical analysis of protein-protein interactions, and See more • Cellosaurus entry for THP-1 See more Webคือตอนนี้กำลังทำสัมมนาเกี่ยวกับการตรวจสอบสารสกัดสมุนไพรที่มีฤทธิ์ยับยั้ง P. acnes เลยอยากรบกวนถามเกี่ยวกับ THP - 1 cell คืออะไร มีหน้าที่เหมือน T - helper ...

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Web1. To revive THP-1 and Jurkat cells from frozen liquid N 2 stocks, warm RPMI-1640 supplemented with FBS (10%, complete media) to 37 °C in a water bath.. 2. Thaw the capped frozen vial containing cells in a water bath at 37 °C.Note: Only a partial thaw is sufficient, and a small lump of ice should remain in the vial. Limit the toxicity of the DMSO (5%) … WebMar 4, 2024 · Abstract Results obtained showed that infection with HCMV prevented the death of THP-1 cells treated with DOX in both active and latent forms of infection. In the presence of mTOR inhibitors (rapamycin and Torin2), the sensitivity of the infected cells to DOX was restored. Rapamycin inhibited the expression of the HCMV protein IE1-p72 and …

WebCytogenetic and molecular analysis of the acute monocytic leukemia cell line THP-1 with an MLL-AF9 translocation. Genes Chromosomes Cancer 29:333-338(2000) DOI= … WebMay 25, 2024 · A previous study showed that the expression of pro-inflammatory cytokines differs between THP-1 cells and PMA-differentiated THP-1 cells. 11 To further investigate whether these differences depend on the LPS concentration in THP-1 cells and PMA-differentiated THP-1 cells, the expression levels of mRNA and protein were analyzed …

WebNov 18, 2016 · Using the in vitro (THP-1 cells) and ex vivo (primary monocytes) approach side-by-side allowed us to make informed judgments on the usefulness of THP-1 cells as a model for primary monocytes. For example, monocytes, when compared with THP-1 cells, are far more responsive to LPS. The remarkable LPS responsiveness of human peripheral … WebJan 12, 2024 · Once the cells are suspended, seal the tube, invert a few times, and then divide the cells into the freezing vials. Transfer the vials to your equipment for freezing. Do Not Leave Your THP-1 Cells in the -80°C …

WebInvivoGen offers several engineered THP-1 cell lines: THP1 NF-κB cells were specifically designed for monitoring the NF-κB signal transduction pathway in a physiologically relevant cell line. They feature either the SEAP or the Lucia luciferase reporter gene. THP1-Dual™ cells allow the simultaneous study of the NF-κB pathway, by monitoring ...

WebMar 14, 2024 · THP-1 cells differentiated with phorbol 12-myristate 13-acetate (PMA) are widely used as a model for function and biology of human macrophages. However, the conditions used for differentiation, particularly the concentration of PMA and the duration of treatment, vary widely. Here we compare several differentiation conditions and compare … christina tonkin anzWebTHP-1 cell line (ATCC TIB-202) is commonly used to study monocyte and macrophage activities, functions, innate immune mechanisms and signaling pathways. This luciferase reporter cell line was derived from parental line TIB-202 by stably expressing firefly luciferase gene (luc2) under control of a NF-κB promoter. This cell line was established … la tinka cotejarWebPublished protocols using the promonocytic cell line THP-1 have tended to result in cells that closely resemble classically-activated macrophages, differentiated in IFNγ and LPS. … christina saltiWebTHP1-defASC cells are derived from THP-1 human monocytic cells. THP-1 cells are the most commonly used model cell line for the study of inflammasome activation as they express high levels of NLRP3, ASC and pro-caspase-1. THP1-defASC cells express negligible levels of ASC but express native levels of NLRP3 and pro-caspase-1. These cells are ... la tinka loteriasWebJan 12, 2024 · Once the cells are suspended, seal the tube, invert a few times, and then divide the cells into the freezing vials. Transfer the vials to your equipment for freezing. Do Not Leave Your THP-1 Cells in the -80°C Freezer for More Than 24 Hours. THP-1 cells do not like being in the -80°C freezer! I cannot stress this point enough. la tinka resultadosWebTHP1-Null cells are derived from THP-1 human monocytic cells, that represent the most commonly used model cell line for the study of inflammasome activation as they express … christina tillmannWebNov 5, 2024 · Immortalized cell lines have long been used as model systems to systematically investigate biological processes under controlled and reproducible … christina vassell